7BIO

7BIO is a 3′ substituted kinase inhibitory bis-indole, which functions as a caspase independent nonapoptotic cell death inducer. Interestingly 7BIO did not inhibit the activity of CDKs and GSK-3, which is the target of many other kinase inhibitory bis-indoles. In addition, research suggests that kinase inhibitory bis-indoles, also known as indirubins, do not inhibit CDK and GSK-3 when there are substitutions on position 7 via steric hinderance. Alternate studies indicate that 7BIO can greatly reduce mitochondrial transmembrane potential. Furthermore, 7BIO causes the dilation of the Endoplasmic Reticulum and acute cytoplasmic destruction which isolated nuclei with DNA fully intact. 7BIO is also a potent inhibitor of Aurora kinase B and C.