Glycyl-L-arginine p-nitroanilide dihydrochloride

Gly-L-Arg-pNA is a chromgenic substrate for Dipeptiylpeptidase I (DPP I; cathepsin C) and DPP II or P. gingivalis dipeptidyl peptidase. It can furthermore be used as building block for test substrates for Coagulation Factor Xa and XIIa, t-Plasminogen Activator (tPAS) and u-Plasminogen Activator (uPA), Envelysin, C1 Esterase Inhibitor, Trypsin, Matriptase, horseshoe crab clotting enzyme and many others. References: 1. Dipeptidyl peptidase with strict substrate specificity of an anaerobic periodontopathogen Porphyromonas gingivalis; S. Fujimura, K. Hirai and Y. Shibata; FEMS Microbiol Lett 2002; 209: 127-31. 2. The preparation and properties of immobilised dipeptidyl-aminopeptidase I (cathepsin C); D. W. Hutchinson and A. Tunnicliffe; Biochim Biophys Acta 1987; 916: 1-4. 3. Human tissue factor pathway inhibitor fused to CD4 binds both FXa and TF/FVIIa at the cell surface; K. Riesbeck, A. Dorling, G. Kemball-Cook, J. H. McVey, M. Jones, E. G. Tuddenham and R. I. Lechler; Thromb Haemost 1997; 78: 1488-94. 4. Physiological fIXa activation involves a cooperative conformational rearrangement of the 99-loop; K. Sichler, E. Kopetzki, R. Huber, W. Bode, K. P. Hopfner and H. Brandstetter; J Biol Chem 2003; 278: 4121-6. 5. Structural mapping of the active site specificity determinants of human tissue-type plasminogen activator. Implications for the design of low molecular weight substrates and inhibitors; M. Renatus, W. Bode, R. Huber, J. Sturzebecher, D. Prasa, S. Fischer, U. Kohnert and M. T. Stubbs; J Biol Chem 1997; 272: 21713-9. 6. Current diagnostic possibilities in hereditary angioedema and acquired angioedema; W. Dick and W. Cullmann; Immun Infekt 1985; 13: 113-8. 7. Chromogenic substrates for horseshoe crab clotting enzyme. Its application for the assay of bacterial endotoxins; S. Iwanaga, T. Morita, T. Harada, S. Nakamura, M. Niwa, K. Takada, T. Kimura and S. Sakakibara; Haemostasis 1978; 7: 183-8.