HNHA

HNHA is a cell-permeable inhibitor of HDAC (histone deacetylase) activity (IC₅₀ = 100 nM). HNHA induces histone hyperacetylation and p21 transcription with concomitant inhibition of cell cycle progression (IC₅₀ ~ 7.5 μM) in human fibrosarcoma HT1080 cells. Another function of HNHA is blocking retinal neovascularization and choroidal angiogenesis in mice. HNHA also inhibits the growth of human umbilical vein endothelial cells (HUVECs) and prevents tube formation and migration of HUVECs in response to vascular endothelial growth factor (VEGF).