pp60v-src Autophosphorylation Site, Tyrosine Kinase Substrate

pp60v-src Autophosphorylation Site, Tyrosine Kinase Substrate Protein is a tyrosine kinase substrate with the ability to phosphorylate. Residues 2-12 in this peptide correspond to the sequence of the reported site of tyrosine phosphorylation (i.e. aa 412-422) in the Rous sarcoma virus (RSV)-encoded transforming protein pp60v-src. This substrate sequence, RRLIEDNEYTARG, was originally described as an EGFR (epidermal growth factor receptor) substrate in A431 cell extracts (K_m=0.3-0.6 mM). In vitro tyrosine phosphorylation of the peptide is stimulated by EGF (epidermal growth factor). The compound has shown an ability to reverse the kinase process much like DTT (1,4-Dithiothreitol) which has resulted in the increased activity of the insulin receptor/kinase.