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3,3',5,5'-tetramethylbenzidine, free base

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Formula: C16H20N2
CAS: 54827-17-7

Identification

Structural Formula
3,3',5,5'-tetramethylbenzidine, free base
CAS:
54827-17-7
EINECS:
259-364-6
Molecular Formula:
C16H20N2
MDL:
MFCD00007748
Synonym:
3355tmb ; tmbz ; tmb/h ; tmb ; tmb quick ; tmb-b ; tmb easy ; tmb/m ; tmb-e ; bm blue


Description

Substrate for colorimetric Peroxidase detection. Colorless TMB solution turns blue upon peroxidase reaction with hydrogen peroxide. 1. Holland V.R. et al, A Safer Substitute for Benzidine in the Detection of Blood. Tetrahedron 1974; 30: 3299-3302; 2. Pujol F.H. et al., A Double Sandwich Monoclonal Enzyme Immunoassay for Detection of Hepatitis B Surface Antigen. J Immunoassay 1993; 14: 21-31.


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Indofine Chemical Co, Inc.
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Iris Biotech GmbH
LS-3250
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Prices & Availability




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Properties for 3,3',5,5'-tetramethylbenzidine, free base

Molecular Weight:
240.34 g·mol−1
Density:
1
Melting point:
168-171 °C(lit.)
Boiling point:
100 °C
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Hazard Statements

GHS07

GHS03
Harmful
  • Acute toxicity (oral, dermal, inhalation), category 4
  • Skin irritation, categories 2, 3
  • Eye irritation, category 2A
  • Skin sensitization, category 1
  • Specific target organ toxicity following single exposure, category 3
    - Respiratory tract irritation
    - Narcotic effects
  • Not used
  • with the "skull and crossbones" pictogram
  • for skin or eye irritation if:
  • the "corrosion" pictogram also appears
  • the "health hazard" pictogram is used to indicate respiratory sensitization

Safety & Transport Information

Risk Codes:
R22 - Harmful if swallowed
R36/37/38 - Irritating to eyes, respiratory system and skin
R36/38 - Irritating to eyes and skin
R41 - Risk of serious damage to eyes
Safety Codes:
S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
S36 - Wear suitable protective clothing
S36/37 - Wear suitable protective clothing and gloves
S39 - Wear eye/face protection
Hazard Symbols:
Xi, Xn
Hazard symbol Xn Hazard symbol Xi
Storage temperature :
2-8°C
RID/ADR certification :
UN 2796 8 / PG 2
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Alternative Distributors of [3,3',5,5'-tetramethylbenzidine, free base]

Producers or manufacturers change the product range from time to time. Currently there are no other manufacturers known.
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MSDS:
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