TLCK hydrochloride

TLCK hydrochloride (TLCK) is an active site-directed agent that irreversibly inhibits trypsin-like serine proteases such as granzyme D and tryptase. Cysteine proteases and other enzymes may be inhibited by TLCK through non-selectively interactions with thiol groups. To prevent proteolytic degradation, TLCK may be employed in protein purification protocols. TLCK has been shown to selectively inactivate clostripain obtained from C. histolyticum. Also, TLCK has been observed to inhibit other proteases including plasminogen (plasmin), thrombin, papain and some kinases including PKC. The compound has demonstrated Inhibition of LPS-induced expression of COX-2 in macrophages. TLCK hydrochloride is an inhibitor of trypsin.